best methos for high throughput sequencing of ESTs from cDNA libraries

1 post / 0 new
afhass
afhass's picture
best methos for high throughput sequencing of ESTs from cDNA libraries

I am working on a C4 halophyte grass with 7 chromosomes and a genome arround 350Mb. I  have prepared cDNA libraries from stressed plants (root and leaf). I would like to sequence as many as possible of EST. Are the  new methods for sequencing suitable for this application (solexa, 454 ...??? My final goal is to sequence the genome of this plant basing on the high homology with rice (comparision based on 500 ESTs)