why my DNA looks smeary on the undenature acrylamide gel

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lixiaoze
lixiaoze's picture
why my DNA looks smeary on the undenature acrylamide gel

Before to run the DNA-protein binding complex on the gel, I want to see how my DNA looks like on the gel(gel shift assay).

The DNA looks fat and little smeary not sharp. I used TBE buffer and 29:1 acrylamide.

I tried making 5 gels so far with different new made TBE. Could anyone tell me what's wrong with it?

Really appreciate it.