Hi all. I am having problems with a step in the Wizard Plus SV Minipreps system for isolating plasmids by Promega. After cell lysis ( as well as protease inhibitor and subsequent neutralization steps), you have to spin down the cell lysis solution in order to separate gDNA precipitate from the plasmid (I am getting good lysis as noted by the "stringy/sticky" appearance). After I have spun down the tubes, I get a great pellet, but when I pipet off the supernatant I still see "debris" getting sucked up into my pipetman tip (although I am not disturbing the main pellet at the bottom). I have tried adding 5 more minutes to the centrifugation step and have also added a second centrifugation step to try and pellet the stuff that I manage to suck up in pipetman. Should I spin longer to get the other debris/floaties into the pellet? Any ideas/suggestions would be greatly appreciated!