This is my first question here. I hope I can intelligibly explain my problem. It is about getting metaphase chromosomes from peripheral blood culture. I can't get metaphase chromosomes on my slides enough. Generaly there just a few metaphase per slide(with 5 drop) or there is no metaphase. I don't know where I make mistake. It is so complicated so it could be anything. If someone can help me about this problem I can give more details like a protocol that I follow and the images that I get from the inverse microscop.
Getting metaphase chromosomes is the preliminary step for further analysis like FISH and chromosomal analysis. But first I should solve this problem. Help me please:)