We are currently runnining an assay, where the radiolabel splits into two from the reaction, with the radioactive bit in the aqueous phase, and the non – radioactive bit in the organic phase.
I now need to quantify the amount of radiation in the aqueous phase (in nM).
I know how to work out radiactive concentrations (from specific activity and cpm).
But, am I right in assuming that the specific activity of the split radiation molecule will be the same as the parent radiation molecule I put in the assay.
I need the specific activity to work out my concentrations.
Sorry if this is a daft question.
Thank you for your help!