protein isolation

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Keerthi
Keerthi's picture
protein isolation

 Dear All

I am trying to isolate protein from frozen human pancreatic tissue. I have used the generic protocol but it has not yielded to the concentration of my desire. Is there a pancreatic specific protocol? Please do let me know.

Sami Tuomivaara
Sami Tuomivaara's picture
Keerthi,

Keerthi,

Here's one protocol:

Börner et al. (2009) BioTechniques 46: 297–304.

Cheers,

Keerthi
Keerthi's picture
Thanks a lot.. i tried this

Thanks a lot.. i tried this protocol but it did not owrk very well for me.... thnks for the help!!!!