How can I figure out how much enzyme (concentration) I need for a given experiment. For example, I want to find out how much enzyme I need to degrade cell walls of a piece of plant tissue. I want to use the optimal amount of reagent and complete the process in a reasonable amount of time. How can I find out how much enzyme I require without running controlled experiments to test for best concentration and wasting reagents, which defeats the purpose. A general equation for this process? Anything?? Thanks!