ANGIOGENESIS IN CANCER AND VASCULAR DISEASE

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Fraser Moss
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ANGIOGENESIS IN CANCER AND VASCULAR DISEASE

ANGIOGENESIS IN CANCER AND VASCULAR DISEASE

www.med.miami.edu/mnbws

February 4-8, 2006

Wyndham Miami Beach Resort Hotel

Awardees: Kari Alitalo, Peter Carmeliet, Judah Folkman, Rakesh Jain

Topics: Molecular Mechanisms of Angiogenesis, Stem Cells and Vascular Regeneration, Tumor Angiogenesis & Vascular Disease, Lymphangiogenesis, Angiogenesis Inhibition, Emerging Technologies, and Therapeutic Angiogenesis

Sponsored by the University of Miami and Nature Publishing Group

Organized by the University Biochemistry & Molecular Biology Foundation, Inc., The Sylvester Comprehensive Cancer Center and Nature Medicine, Nature Cell Biology and Nature Biotechnology

swdbell
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I have some question about

I have some question about angiogenesis method. I have a problem in experiment.
I do Spheroid assay for 3D spheroid by Hanging drop technique. Using matrix by collagen from rat tails so my matrix is not clear   I think it participate of collagen,   because it has a white fibrous whan see in well and debris in microscope.
From this problem impact for spheroid is not good,not circle,       cell of this expose form there in next time so don't see abnormal shape in first day.         I think this problem happens form collagen hasn't well of polymerization.
How can do it ?       I do collagen on ice 3-5 minitue.

Thanks you

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