how much of 10mM stock is used to make 10uM final concentration

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VT
VT's picture
how much of 10mM stock is used to make 10uM final concentration

Hello,

I have 10mM stock solution and have to add to the culture of 500uls to make it 10uM final concentration of the stock to pulse the cells. SO how much I should take from the stock in order to make 10uM final concentration?

Thanks,
VT

vanshita
vanshita's picture
Hi VT

Hi VT
In order to make 10uM final concentration for the  total volume 500ul, you should take 0.5ul from stock 10mM but as the volume is too less so error of chances can increase. so first dilute your stock, 10 times to make it 1mM (5ul of 10mM stock  + 45ul of water) and then take 5ul from stock of 1mM and 495ul of media.