Transfection of HEK cells

1 post / 0 new
swati9
swati9's picture
Transfection of HEK cells

I was trying to Transfect HEK cells in Calcium phosphate and repeated 3 times,but with the transfected cells couldn't get desired result as when ever I'm doing Western blot,I got brighter and wider bands for negative control(non transfected cells) also  got numerous (more than 10) bands which were nonspecific.I've followed strict washing protocol still couldn't avoid the problem completely.can anyone tell me please - what could be the reason and how to avoid  ,I'm really new and inexperienced