need help

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MD Hossain
MD Hossain's picture
need help

Hello,
I need some suggestions regarding how to make put sample solution into the dialysis tube. I tried to to open the dialysis tube but I could not. In some papers they suggest to wash in water, this will help to open the tube so that sample solution can easily be put into the tube. However, my experiment is in organic solvent. I just want to remove CHCl3 solvent from block copolymer micelle in toluene solvent. Could anybody suggest me about it?
Regards
Hossain

Ivan Delgado
Ivan Delgado's picture
Hi MD,

Hi MD,
Placing a solution into dialysis tubing can be tricky. If you are having problem you may want to consider getting tubing that has a larger diameter. Alternatively submerge the tubing in water, that should help you get it open. Rubbing both sided of the tip of the tube, much in the same way you do when trying to open plastic grocery bags typically works. 
If all this fails you may want to consider using a Slide-A-Lyzer (from Pierce) instead of dialysis tubing. The really nice thing about these dialysis cassettes is that you do not have to play around trying to get them set up. All you do is open one, push your solution into the cassette using a syringe, and you are ready for dialysis. 
Good luck

RLS
RLS's picture
 I hold the tubing under

 I hold the tubing under running distilled water, rubbing one end until it comes open. Then you can run water down into the tubing, but as Ivan said, dunking it in water and rubbing on the opening at one end should also get it apart. Then you can run water through it to rinse it out. Some people also boil it or autoclave it in water before use.

MD Hossain
MD Hossain's picture
Hi Ivan,

Hi Ivan,
Many thanks for your suggestions. I am really pleased to receive your answer and I believe that it will be very helpful specially using a slide-A-Lyzer instead of dialysis tubing. I did not know about it. However, thanks again for your valuable suggestions.
Regards
MD Hossain
Pukyong National University
Busan, Korea
 
Hi MD,
Placing a solution into dialysis tubing can be tricky. If you are having problem you may want to consider getting tubing that has a larger diameter. Alternatively submerge the tubing in water, that should help you get it open. Rubbing both sided of the tip of the tube, much in the same way you do when trying to open plastic grocery bags typically works. 
If all this fails you may want to consider using a Slide-A-Lyzer (from Pierce) instead of dialysis tubing. The really nice thing about these dialysis cassettes is that you do not have to play around trying to get them set up. All you do is open one, push your solution into the cassette using a syringe, and you are ready for dialysis. 
Good luck

g a
g a's picture
have you thought of using

have you thought of using small wonder dialyzers????
they are just like eppedorfs or other tubes..... u put your sample and invert it for dialysis againsta solution and after you are done you just make it straight and collect your samples. these are the easiest to handle and very effective.
also you can try opening a dialyzing tubing with the 24g needle.
but wonder dialyzers are the most upcoming ones
happy Dialyzing
Gaganjot Singh

RLS
RLS's picture
 Hi Argerine,

 Hi Argerine,
 
Can you tell us what company/ies make these dialyzers?
 
Thank you,
Lynn

g a
g a's picture
Dear Lynn

Dear Lynn
Pragti biosciences supplies these dialyzers