Lysis buffer protocols

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haileyp
haileyp's picture
Lysis buffer protocols

I work in a molecular ecology lab and my boss has given me some White Tailed Deer lymph node samples from which to extract DNA. The protocol that I'm using requires me to make a 1x Lysis buffer. The recipe that was recommended to me looks like this:
1.21g Tris base
37.22g EDTA
5.00g SDS
All of this in 1 L of water
But I have not been able to get this mixture to go into solution with constant stirring with a stir bar, or with heating. If anyone has any suggestions for a way to keep all of this in solution or has another lysis buffer to recommend I would appreciate it!

osmcen
osmcen's picture
Adjust th pH. EDTA will not

Adjust th pH. EDTA will not go into solution aat low pH.
Good luck