As a graduate student and post-doc, I never really got statistics. It all seemed like confusing math that Id rather not think about. However as I grew further in science and tried to publish papers on my own and began to direct graduate students of my own, I realized I had to figure it all out.

At the Society for Neuroscience conference in Washington, D.C. I met one of the founders of GraphPad. Prism is the program I use mostly for stats, so I talked the poor guy's head off.

Anyway he turned me onto his blog on the Graph Pad website. I love this article he wrote and got his permission to post it here.

The original blog can be accessed here

[b]Is it better to plot graphs with SD or SEM error bars? [b]

(Answer: Neither) FAQ# 201

There are better alternatives to SD and SEM, depending on your goal.

If you want to show the variation in your data:

If each value represents a different individual, you probably want to show the variation among values. Even if each value represents a different lab experiment, it often makes sense to show the variation.

With fewer than 100 or so values, create a scatter plot that shows every value. What better way to show the variation among values than to show every value? If your data set has more than 100 or so values, a scatter plot becomes messy. Alternatives are to show a box-and-whiskers plot, a frequency distribution (histogram), or a cumulative frequency distribution.

What about plotting mean and SD? The SD does quantify variability, so this is indeed one way to graph variability. But a SD is only one value, so is a pretty limited way to show variation. A graph showing mean and SD error bar is less informative than any of the other alternatives, but takes no less space and is no easier to interpret. I see no advantage to plotting a mean and SD rather than a column scatter graph, box-and-wiskers plot, or a frequency distribution.

Of course, if you do decide to show SD error bars, be sure to say so in the figure legend so no one will think it is a SEM.

If you want to show how precisely you have determined the mean:

If your goal is to compare means with a t test or ANOVA, or to show how closely our data come to the predictions of a model, you may be more interested in showing how precisely the data define the mean than in showing the variability. In this case, the best approach is to plot the 95% confidence interval of the mean (or perhaps a 90% or 99% confidence interval).

What about the standard error of the mean (SEM)? Graphing the mean with an SEM error bars is a commonly used method to show how well you know the mean, The only advantage of SEM error bars are that they are shorter, but SEM error bars are harder to interpret than a confidence interval.

Whatever error bars you choose to show, be sure to state your choice.

If you want to create persuasive propoganda:

If your goal is to emphasize small and unimportant differences in your data, show your error bars as SEM, and hope that your readers think they are SD

If our goal is to cover-up large differences, show the error bars as the standard deviations for the groups, and hope that your readers think they are a standard errors.

This approach was advocated by Steve Simon in his excellent weblog. Of course he meant it as a joke. If you don't understand the joke, review the differences between SD and SEM.

from the GraphPad Prism website

http://www.graphpad.com/faq/viewfaq.cfm?faq=201

Thank you for the post, it will definitely help everyone.

Here is a related paper which describes about the Error bars & statistics.

"Error bars in experimental biology". Geoff Cumming, Fiona Fidler,and David L. Vaux.The Journal of Cell Biology, Vol. 177, No. 1, April 9, 2007, pp 7-11.

http://www.jcb.org/cgi/doi/10.1083/jcb.200611141