Transfection reagent for cotransfection of plasmids and siRNA?

6 posts / 0 new
Last post
Edward Dougherty
Edward Dougherty's picture
Transfection reagent for cotransfection of plasmids and siRNA?

Anyone out there know a good transfection reagent for co-transfection of plasmids and siRNA? In particular, any reagent good for transfection of CV-1 or COS-7 cells would be most helpful.

Thanks,

Ed

Fraser Moss
Fraser Moss's picture
DharmaFECT Duo or

DharmaFECT Duo or Lipofectamine 2000 work

xangwang
xangwang's picture
Hi Ed,

Hi Ed,

is your transfection question still of actuality ?
I've used a lot Magnetofection and it works very well for co-transfection

xang.

The FFM
The FFM's picture
 Xang

 Xang

Would you mind posting your Magnetofection protocol please?

Put a copy in the Gene silencing protocols section and link back to it in a post here

Thanks!

xangwang
xangwang's picture
Hi !

Hi !

I've put my protocol in the gene silencing protocols section,

www.scientistsolutions.com/a7244-protocol-sirna+and+plasmid+dna+co_transfection+protocol+with+magnetofection.aspx

Hope that will work

xang.

The FFM
The FFM's picture
Excellent! Thank You

Excellent! Thank You