Has anyone had any experience using Ambion's new reverse transfection reagent NeoFX? We have had no success using their positive control GAPDH in MCF-7 cells with this reagent. We are not getting any help from Ambion even though we send them questions. Does anyone know any 'tricks' to getting this to work??? We are using Taqman real time to check transcript levels. Occasionally we get a inhibition in an isolated well but nothing is reproducible.