RNAi promoter

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happy life
happy life's picture
RNAi promoter

Hi
Does any one know the difference between the RNAi expression vector  promoter U6 and H1 .
I plan to order the  pSilencer 5.1 retro retroviral expression vector ,but I have no idea about the choice of promoter . which is better or it just depend the different cases.

Thank you !

Chin Fen Teo
Chin Fen Teo's picture
 Hi happy life,

 Hi happy life,

There is a research article out there comparing these two promoters in mediating gene silencing.

Their conclusion: For that particular type of cells they were using (endothelial), U6 promoter is more superior.

However, I would suggest you to look into the cell line that you will be using in the future and pick one that works better.

Good luck.

happy life
happy life's picture
Hi pippuri

Hi pippuri

Thank so much for your reply .

In fact  I have already  read  some articles including the one you suggested, but I could not get a clear conclusion for them .

As you suggested , it should depend on different cell lines and I need to do some preliminary
experiment of both , but costly.

Chin Fen Teo
Chin Fen Teo's picture
 Hi happy life,

 Hi happy life,

Instead of purchasing both types of vector, one thing that you could do is to dig into the literature and skim through the papers reported any kind of shRNA knockdown experiments using your cell line of interest, and see which promoter is the more popular one.... It may take some time, but it is worth...

Good luck.

happy life
happy life's picture
Hi pippuri 

Hi pippuri 

    Thank you so much for your advice , I will try .