hey guys i have very weird prob with my PCR. well it was working fine for a couple of days and then i stopped working for two weeks and then when i started with it again its not working..i get smears on my gel and no product. what do u think s the prob. even the positive samples dat i got earlier is nt working now.
i discarded all my old reagents and used new vials yesterday but same result? i don't think its the samples cause my friend s using the same samples for his PCR with different primers and it works nicely with clear strong bands.
pls give me some solution pls..thanks..