Should I add DMSO for my PCR protocol, the %CG is around 65.
If i do then how much?
There are many different reagents that can be added to a PCR reaction to get around a high GC content. DMSO is one of them, so is Betaine. One starting point to test DMSO in PCR is 5%. You may want to test other percentages in case 5% does not work.