i get sequence pick very noise.i don't know what is the original reason. my primer is diluted in ultrapure water.if primer is degraded or something else..
I could help you with this. Plese give me information on
1.template for sequencing
2. reaction chemistry- cycle sequencing etc
3. length of expected product
4. purification method used for PCred product
5. annealing temp of the sequencing primer
6. % GC content of the template
7. Explain how exactly the primer was reconstituted for sequencing reaction.
thank u very much.i will give u all data u need.