I'm having a problem to extract genomic DNA from Ganoderma using the conventional method (phenol/chloroform). The protocol used to be good, just don't know why the DNA extracted recently tends to form transparent, gel-like thing when it comes to isoproanol precipitation step and when it is to be dissolved in TE buffer at the final step, it turns to be viscous..
FYI: The DNA extracted will be used for genome sequencing, so we really need good quality DNA
Anybody come across this problem? Or do you have any suggestion on what other protocols can be used for fungal DNA extraction ?