difficult choice between ABI 3500 and Beckman GenomeLab

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genomer
genomer's picture
difficult choice between ABI 3500 and Beckman GenomeLab

Hi people!

Will somebody help me to make the right choice?
The problem sounds like that: I'm going to get sequencer for my genotyping laboratory and I've got 2 options to choose between - ABI 3500 and GenomeLab (from Beckman).

So which one would you recommend to obtain?

Some people say that GenomeLab uses the chemistry that is too fastidious in terms of PCR product purity - due to (probably) poor ability of Beckman proprietary Taq polymerase to incorporate ddNTPs.
That is in contrast to nearly completely "adjusted" Taq polymerase produced by Applied Biosystems.
Again, these are only rumours.

So, does anybody know anything more precise about all this stuff?

Thank you in advance!

Dragon fly
Dragon fly's picture
Hi,

Hi,

If you run Fragment Analysis only, GeXP or CEQ is good enough. If you run Sequencing often, ABI is better. It gives you longer sequences.
Also, Big dye (ABI) is more available  and popular than Wellred (Beckman). Beckman's reagent is too hard to get.

genomer
genomer's picture
Hi, Dragon

Hi, Dragon
Thank you for your reply
Actually, I gonna run FA more often than sequencing, and I've choose already GeXP.
So, your reply is exactly on point.

Max