my gene of intrest is of 2.3kb which is to be amplified with a A, B primers .but there is very faint band coming just above 2.5kb. the same template , i used to amplify 400bp segment with primers A, C primers. the result was good.
do i need to use faint band pcr product as template for pcr.
but i have come to know that sometimes the second pcr product would not be our desired segment.
is it true.
plz find me a solution.
i need clone this 2.3 band.
thanks in advance.