We bought Lihtcycler 480 our lab. I want to screen CaMV 35S promoter in RR soya. Forexample I have got %0.1 RR soya.My sample is 245,5 ng. If 1 ng soya DNA 598 kopy, My calculate is 245x598= 146,809 , %0.1 x146,809=146 kopy. ?s ?t true? ?f is it true, How can I prepare standart curve. I want to prepare 100, 50,20,10,5 copy. How can I do.Would you like to respond me question to detail format.