I'm working on a qPCR protocol where I'm trying to get optimal parameters for 9 different primer pairs per 96-well plate using bacterial genomic DNA as template. I'm currently using SYBR green for detection, since hydrolysis probes would be a little pricey at this point. My problem is that I'm getting too much background in some of my primer pairs' no-template controls, typically at about the same Ct as the 10 fg level of their respective standard curves. This is equivalent to around 2 cells worth of gDNA, which isn't a lot, but tell that to my boss. Anyway, I'm looking for some ideas for knocking down the background, keeping in mind that I have to strike a balance for all 9 primer pairs.