Hello all. I am back again with a new question.
I started my RT-PCR but I wasconfused with somthing..
I do have 2 house keeping gene, two tissue and4 genes (8 primers)
I want to do 4 dilution and undiluted sample
which sample should assigned as standered and unkown to have a standered curve
and what is the intial concentration for each sample diluted and undiluted as I was asked by the program
I need help please as soon as possible
do you know any helpful website as well
thank and all