I made all my analyses with a standard curve for beta-actin (control) with purified PCR product amplified from a cell culture gDNA. Afterwards I realized that the cell I used is from mouse and I analyze human samples. I borrowed cells culture from someone. One primers differ 2 bases and other only one base pair. Obviously the amplicons TMs are different too. But the efficiency range from 100 to 96%. So can I use this curve to calculate actin copies in my samples? Or will I have to do it all again?