qPCR Problems

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llarissa
llarissa's picture
qPCR Problems

Before using this primer pair, I made ??an efficiency curve using dilutions of a single sample. The curve was perfect with 98% efficiency.
Now I'm using the same primer pairs to validate some samples. However each sample shows differents melting curves for the same primer pair. This samples are the same just different biological replicates.
this happens only with this single primer I run a constitutive gene for this same samples and the melting curves are ok!