I am interested in designing primers to determine amplification or deletion of my gene in the genome. On e-PCR (NCBI) I get results in my "genomic section" of the same species I am interested in. Now most of these seem to locate to the same chromosome, however, I was wondering how I determine whether they are infact of my gene of interest, or other genes targeted by my PCR primer set. It does not show any results in the "cross-references" section that is not the orginal gene of interest.
However, I do have another gene that does show "cross-reference" to another gene in all primers designed by e-PCR. What would be an appropriate methodology for designing primers for this gene. Thank you.