qPCR NEWSLETTER

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qPCR NEWSLETTER

qPCR NEWSLETTER

Dear researcher,
dear Gene Quantification page reader,

Our newsletter informs about the latest news in quantitative real-time PCR (qPCR and qRT-PCR), which are compiled and summarised on the Gene Quantification homepage. The focus of this newsletter issue is:

- qPCR 2007 Event - Symposium & Exhibition & Workshop
- Focus of this month => OPTIMISATION
- New NARG study 2007
- GOOGLE qPCR Alert
- TATAA courses in 2007

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qPCR 2007 Event - 3rd International qPCR Symposium & Industrial Exhibition & Application Workshop
26th - 30th March 2007, in Freising-Weihenstephan,
Technical University of Munich, Physiology-Weihenstephan, Germany

http://qpcr2007.gene-quantification.info/

On behalf of the Organisation Committee and the Scientific Board it is great pleasure to invite you to the 3rd International qPCR Symposium & Industrial Exhibition & Application Workshop to be held at the Center of Life Science in Freising Weihenstephan, Technische Universitt München (Germany). The great international interest in the previous meetings (qPCR 2004 and qPCR 2005) with up to 465 participants in each event from over 40 countries, and 30 international companies in the qPCR Industrial Exhibition led us to the decision to repeat it in spring 2007.
We have set the date for the qPCR 2007 Event 26 30th March 2007. The event location is the central lecture hall complex and the foyer at TUM (Technical University of Munich) in Freising Weihenstephan, Germany. The TUM and the Biotech region around Munich is part of the largest Biotech cluster in Europe, located close to the Munich airport in the heart of Bavaria.
Leading academic researchers and industrial contributors in the field will be participate in the symposium, which will be an arena for fruitful discussions between researchers of different backgrounds. Here you will find a list of the Symposium talks (preliminary). The Symposium Talks, various Poster Sessions, Industrial Exhibition and three associated TATAA Application Workshops offer an overview of the present knowledge and future developments in qPCR technology and its wide applications.

The qPCR 2007 Event is structured with three parts:

1. qPCR Symposium taking place March 26 28, including various Talk and Poster sessions
2. A parallel qPCR Industrial Exhibition taking place March 26 28
3. Followed by three qPCR TATAA Workshops taking place March 29 30

CALL for scientific contributions: TALK and POSTER presentations

=> The focus of the qPCR 2007 Event will be on single-cell qPCR, and microRNA / siRNA quantitative RT-PCR applications
=> Final deadline for abstract submission ( Talks and Posters ) => 31st January 2007
=> Please register and submit your abstract here => https://www.wzw.tum.de/conftool/

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qPCR 2007 sessions Prime session: Single-cell qPCR
all around single-cell qPCR, pre-amplification techniques, laser micro dissection, sub-cellular PCR, micro-manipulation of cell clusters, cellular micro injection,

Prime session: microRNA siRNA Applications
microRNA extraction, qRT-PCR technologies to detect microRNA, siRNA applications in combination with real-time RT-PCR, microRNA targets and microRNA precursors, new siRNA manipulation and microRNA technologies, .....

Immuno qPCR
development, establishment, optimization of immuno-qPCR, innovative immuno qPCR applications, ..

New diagnostic applications with real-time PCR
new quantification methods, new dyes and probe technologies, SNP analysis, high resolution melt applications, Marker Genes (diagnostic, prognostic and therapeutic markers on DNA and RNA level), .......

High throughput quantitative PCR
96 well and 384 well applications, new high throughput platforms, SNP application, gene expression real-time RT-PCR arrays, quantitative multiplexing, ..

Pre-analytical Steps
sampling technologies, DNA / RNA purification, DNA / RNA quality control, Reverse Transcription, RT quality control, external references, ..

qPCR NOS Session - Normalization & Optimization & Standardization
new types of normalization, one vs. multiple reference genes, genomic DNA as standard, external standards, optimization of the real-time PCR, inhibition of negative effects, optimization of real-time PCR efficiency, multiplexing, establishment of DNA / RNA standards, inter-run standards, national and international studies on qPCR standardization, quantification strategies, ........

qPCR BioStatistics & BioInformatics
software applications, calculation of relative expression, data mining, primer and probe design, real-time PCR efficiency determination, CP determination from amplification response curves by mathematical modelling, raw data analysis, statistics in real-time PCR, data management, 3D data visualization, ........

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New publications: http://www.gene-quantification.de/real-time.html#newpub

Quantification of mRNA using real-time RT-PCR
Tania Nolan, Rebecca E Hands & Stephen A Bustin
Nature Protocols Vol. 1, No. 3, (2006) p1559 - 1582

The real-time reverse transcription polymerase chain reaction (RT-qPCR) addresses the evident requirement for quantitative data analysis in molecular medicine, biotechnology, microbiology and diagnostics and has become the method of choice for the quantification of mRNA. Although it is often described as a gold standard, it is far from being a standard assay. The significant problems caused by variability of RNA templates, assay designs and protocols, as well as inappropriate data normalization and inconsistent data analysis, are widely known but also widely disregarded. As a first step towards standardization, we describe a series of RT-qPCR protocols that illustrate the essential technical steps required to generate quantitative data that are reliable and reproducible. We would like to emphasize, however, that RT-qPCR data constitute only a snapshot of information regarding the quantity of a given transcript in a cell or tissue. Any assessment of the biological consequences of variable mRNA levels must include additional information regarding regulatory RNAs, protein levels and protein activity. The entire protocol described here, encompassing all stages from initial assay design to reliable qPCR data analysis, requires approximately 15 h.

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Optimisation: Optimisation.gene-quantification.info/

Evaluation of probe chemistries and platforms to improve the detection limit of real-time PCR.
Reynisson E, Josefsen MH, Krause M, Hoorfar J.
J Microbiol Methods. 2006 Aug;66(2):206-16.

Optimized real-time quantitative PCR measurement of male fetal DNA in maternal plasma.
Zimmermann B, El-Sheikhah A, Nicolaides K, Holzgreve W, Hahn S.
Clin Chem. 2005 Sep;51(9):1598-604.

Standardizing the standards.
Quackenbush J., Mol Syst Biol. 2006;2:2006.0010.
The nice thing about standards is that there are so many to choose from. Andrew S Tannenbaum

Evaluation of dual-labeled fluorescent DNA probe purity versus performance in real-time PCR.
Yeung AT, Holloway BP, Adams PS, Shipley GL.
Biotechniques. 2004 Feb;36(2): 266-70, 272, 274-5.

Determination of allele frequency in pooled DNA: comparison of three PCR-based methods.
Wilkening S, Hemminki K, Thirumaran RK, Bermejo JL, Bonn S, Forsti A, Kumar R.
Biotechniques. 2005 Dec;39(6): 853-8.

EQUAL-quant: an international external quality assessment scheme for real-time PCR.
Ramsden SC, Daly S, Geilenkeuser WJ, Duncan G, Hermitte F, Marubini E, Neumaier
M, Orlando C, Palicka V, Paradiso A, Pazzagli M, Pizzamiglio S, Verderio P.
Clin Chem. 2006 Aug;52(8):1584-91

Diagnostic PCR: validation and sample preparation are two sides of the same coin.
Hoorfar J, Wolffs P, Radstrom P.
APMIS. 2004 Nov-Dec;112(11-12):808-14.

Risk assessment of false-positive quantitative real-time PCR results in food, due to detection of DNA originating from dead cells.
Wolffs P, Norling B, Radstrom P.
J Microbiol Methods. 2005 Mar;60(3):315-23.

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NARG 2007 NARG 2007 - Real-Time PCR Survey (Study Open)

http://www.gene-quantification.de/optimization.html

The Nucleic Acid Research Group (NARG) of the Association of Biomolecular Resource Facilities (ABRF) invites anyone who uses Real-Time quantitative PCR (qPCR) to participate in our on-line survey. The aim of the survey is to determine the current status of real-time PCR technology in laboratories around the world, particularly core laboratories. Your answers will help us "take the pulse" of the real-time qPCR community. Submissions are anonymous and results will be freely available via a "web poster". This survey will be open until February 2, 2007. Results will be presented at the ABRF 2007 annual meeting in Tampa Bay, FL, Mar 31-Apr 3, 2007 and will be available "on line" by May 1, 2007. We think it will be worth your time to participate in this study!

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TATAA Biocenter Germany - qPCR Application workshops

http://tataa.gene-quantification.info/

At the TATAA Biocenter Germany we offer qPCR application workshops, the 3-day Core Module and a 2-day Biostatistics Module. qPCR courses are held in regularly in Gteborg, Sweden in English and in Freising-Weihenstephan, Germany in German and English. Depending on the occasion the workshop language and the different prices may apply. Further customized workshops and specialized trainings will be held as well across Europe and world-wide. TATAA Biocenter Germany courses are held in cooperation with the Institute of Physiology, located at the Technical University of Munich, in Freising-Weihenstephan, near Munich, very close to the Munich Airport (MUC). For more information and to register for the qpCR application workshops, please see our web page: http://tataa.gene-quantification.info/

Course Occasions 2007: Please register here: http://www.tataa.com/

- 29th Jan - 2nd Feb 2007 (in German) 3-day Core module and 2-day BioStatistics Module
- 29th Mar - 30th Mar (in English) at the qPCR 2007 event http://qpcr2007.gene-quantification.info/
- 7th May -11th May (in German) 3-day Core module and 2-day BioStatistics Module
- 2nd - 6th July (in English) 3-day Core module and 2-day BioStatistics Module

three workshops for 2 days (in parallel)
- 2-day Core Module
- 2-day BioStatistics Module
- 2-days Sample-prep/Immuno-qPCR mixed Module

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Forward Please send the qPCR NEWS to further scientists and friends who are interested in qPCR and in our Academic & Industrial Information Platform for qPCR.

Best regards,

Michael W. Pfaffl
responsible Editor of the Gene Quantification Pages
http://www.gene-quantification.info

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The qPCR NEWS and the Gene Quantification Pages are educational sites with the only purpose of facilitating access to qPCR related information on the internet. The qPCR NEWS and the Gene Quantification Pages are edited by Michael W. Pfaffl and powered by BioScience Events. Copyright 2005 - 2006 All rights reserved. Any unauthorized use, reproduction, or transfer of this message or its contents, in any medium, is strictly prohibited. Disclaimer & Copyrights are displayed on the homepage www.gene-quantification.com To subscribe or change your e-mail address in qPCR NEWS, and if you would like to receive future issues FREE of charge, please send an e-mail with the subject SUBSCRIBE to mailto:newsletter@gene-quantification.info?subject=SUBSCRIBE