i have a problem in RTqPCR. i tried three different genes of normalization (GAPDH, B-actin and S26) but each time these 3 genes have different values.
i am using stable cell lines (p65,c-Rel and IKB) of jurkat for extraction of RNA.
for making of cDNA i use 1000 ng RNA and use Superscript III reverse transcriptase (invitrogen).
is thre any problem with my RNA samples or stable cell line give different values.
please suggest something for my problem.
problem of normalization gene in RTqPCR