Hi to everyone,
Here is my problem. I have 30 samples equally divided into 3 groups (control (n=10), treatment A (n=10) and treatment B (n=10)). I need to check out expression of 3 different genes, one of them is a reference gene. All my samples will be run in triplicate and here is my dilemma how to setup a 96 -well plate.
I see three options how to run this:
1. run one gene in all samples - one gene/plate
2. run all three genes in one group - one group/plate
3. run all samples and all three genes - one replication/plate
What is the best solution here?