PCR Primers

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Reema0585's picture
PCR Primers


I am a new user here. I need you help in quantitative PCR. I have already chosen the forward and reverse primer but how can I order them? 

and what if i would like in the future to design my own primers how can get the genomic sequence and how can I choose the appopriate ones!

thank you!

Ivan Delgado
Ivan Delgado's picture
Hi Reema,

Hi Reema,

Ordering primers is easy. All you need to do is choose the company you want to get your primers from, IDT, Invitrogen, etc, and simply go to their website and order the primers. 

To get a genomic sequence there are many places where you can get it. One standard one in Genbank. Simply go to their website and search for the DNA sequence you are looking for.

As for designing your own primers, the easiest way is to simply go online and use one of the many free primer design programs out there. IDT has one, BLAST has another one.

Good luck!

Curious George
Curious George's picture

if you are looking for help with assay design, we at TATAA Biocenter offer this service and we have sevral clients in Saudi Arabia already. We also have researchers from Saudi trained at our center. Contact us on: eval(unescape('%64%6f%63%75%6d%65%6e%74%2e%77%72%69%74%65%28%27%3c%61%20%68%72%65%66%3d%22%6d%61%69%6c%74%6f%3a%73%65%72%76%69%63%65%73%40%74%61%74%61%61%2e%63%6f%6d%22%3e%73%65%72%76%69%63%65%73%40%74%61%74%61%61%2e%63%6f%6d%3c%2f%61%3e%27%29%3b')) for more info.

Good luck