i extracted and purified dna from liver samples and got a low 260/230 ratio (~1.0)
I´m gonna detect methylation and perform PCR and sequencing.
The low ratio will affect my analysis?
It depends. Normally a good 260/280 ratio is the most important thing, so if your 260/280 ratio is good (~1.8) then I would not worry too much about it. Still a 260/230 ratio of 1.0 is very low. If possible I would simply extract DNA again.