my DNA 260/230 ratio is too low

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lucascp
lucascp's picture
my DNA 260/230 ratio is too low

Hello,

i extracted and purified dna from liver samples and got a low 260/230 ratio (~1.0)

I´m gonna detect methylation and perform PCR and sequencing.

The low ratio will affect my analysis?

Ivan Delgado
Ivan Delgado's picture
It depends. Normally a good

It depends. Normally a good 260/280 ratio is the most important thing, so if your 260/280 ratio is good (~1.8) then I would not worry too much about it. Still a 260/230 ratio of 1.0 is very low. If possible I would simply extract DNA again. 

lucascp
lucascp's picture
ok, thanks

ok, thanks