I am using Invitrogen super script two step kit . i have problem in repeatability in RTPCR . I get required size band in pilot reaction with good intensity , if i repeat the same RT PCR taking the same RT product as template in more copies to elute the amplicon , i dont get the same good intensity band that i get in pilot reaction . This is happening repeatedly . How to solve this lack of repeatability in RT PCR .
Waiting for quick reply ,
Thanking ypu ,