I have another problem in qPCR. I use GapA primer for my bacteria-orgined samples as internal control. But amounts of expressions are different. It should be same between different samples, shouldn't it? What else can i use as internal housekeeping control for bacteria?
I was wondering something else. Should I have to use any housekeeping gene in my experiments? If I start with equal RNAs, I do not need any house keeping gene, do I?