I wanted to know few things
Will there be variation in the ct values in the real time pcr if cDNA synthesised from different concentrations of RNA are used.
sample labelled as A, 1 microgram of RNA used for cDNA synthesis
sample labelled as A, 0,4 microgram of RNA for cDNA synthesis
when using the cDNA from the above two ways (varying RNA concentration from a single sample) will here be variation in real time PCR?
Its very urgent can anyone help me please