gDNA extraction

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Zam
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gDNA extraction

Hello,

Would anyone please advice me on which protocol/kit to use to extract gDNA from only 0.5x10*6 cells? I need to do HIV breakthrough experiments using qPCR on gDNA extracted from only 5x10*5 cells. I have been using Sigma (GenElute) Kit to extract gDNA but I have been getting low DNA yields (1.5-6 ug og DNA) and depressiong purity, A260/A280 = 1.2-1.4.

Advice, please? 

Kind regards
Zam