elimination of carry-over contamination in PCR?

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bsengez
bsengez's picture
elimination of carry-over contamination in PCR?

Is there anyone doing UDG (Uracil-DNA glycosylase) pre-treatment before qPCR? I want to learn the procedure and the necessaty amount?
Thanks in advance..
Burcu Sengez
 

Ivan Delgado
Ivan Delgado's picture
Have you considered using an

Have you considered using an alternative method, like treating your qPCR master mix with 8-methoxypsoralen? I have used it in the past and it works very well. I do not have any problems with UDG, but I try as much as possible to minimize the number of enzymes present in my qPCR assay. 
Cheers