I am new in q real time pcr and I have problem.
I don't know to calculate a % of gmo based on copy numbers.
I do relative quantification, for example rapeseed, I have one standard curve (based on copy numbers 300000,30000,3000,300,30) for reference genes and another for gmo modification.
I do not know what to do with my quantities.
Or If I have value in copies how to convert it in % of gmo?
DNA copy number and calculating % gmo