DNA contamination in the DNA extraction lab

4 posts / 0 new
Last post
help SA
help SA's picture
DNA contamination in the DNA extraction lab

Hi

I need advise on how to remove DNA contamination in the DNA extraction lab. I usually work in a class II biosafety cabinet.

Ivan Delgado
Ivan Delgado's picture
There are many ways to remove

There are many ways to remove DNA contamination. One is to clean all surfaces with bleach and then ethanol. Another is to install UV lights in the ceiling and turning them on, and leaving them on, during the night. 

Class II biosafety cabinets usually come with instructions on how to clean them. If you do not have those instructions you should contact the manufacturer since it is easy to destroy a cabinet if you use the wrong cleaning solutions. 

Good luck

help SA
help SA's picture
Thank you

Thank you

carolabiotech
carolabiotech's picture
To remove DNA contamination I

To remove DNA contamination I work in an UV cabinet, first I clean with a tissue containing destilled water and then ethanol. then I irradiate with UV for 20 min aprox. I also use a product called DNAzap, it eliminates all the DNA from the surfaces, but you don´t have to use it every time, I use every two weeks aprox.