I need some help setting up a qPCR experiment.
I need to make up dilutions for a qPCR reaction standard curve using the following instructions:
You are provided with a tube that contains 500,000 molecules per microlitre (uL). Set up 4 reaction tubes containing 10^6, 10^5, 10^4 and 10^3 molecules respectively (i.e. 10 fold dilutions). Note that accurate pipetting below 0.5uL is not possible.
Could someone please expain how I can achieve this?