Below is a brief summary of Invitrogen's new SYBR GreenER reagent system. Has anybody used this in their qPCR assays yet? Please post with your comments! Good or Bad?
Molecular Biology Board Moderator
The new SYBR GreenER qPCR reagent system incorporates the latest technology to produce the most reliable gene expression data from real-time qPCR. The SYBR GreenER system is specially formulated to offer the most consistent sensitivity and specificity with:
Novel dsDNA-binding dye-exhibits a brighter signal and less PCR inhibition than the original SYBR Green dye
Highly purified hot-start Taq polymerase-minimizes introduction of DNA contamination
Optimized buffer system-improves specificity and low-copy detection
Uracil DNA glycosylase/dUTP-reduces carryover contamination
More reliable gene expression data
The SYBR GreenER qPCR reagent system delivers superior sensitivity (Figure 1) and specificity across a wide variety of targets. For a comprehensive overview of the SYBR GreenER qPCR reagent system and additional supporting data, visit www.invitrogen.com/sybrgreener.