I would be very happy to learn about the standard and calibrator of Real time PCR. During PCR we usually use calibrator and standard. For example , say I would like to amplify alpha receptor mRNA, for which I need standard alpha mRNA. We use calibratore to normalize between plate to plate. My question is there any certain roles for the strenght diffrences between Calibrator and standard sample. If so how many times strenght differences between this two preparation is better and why?