Absolute standard curves in qRTPCR

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pili
pili's picture
Absolute standard curves in qRTPCR

Hello!
I am trying to perform an absolute standard curve to study gene expression of certain proteins. How can I get single-stranded sense strand oligodeoxynucleotides (that are an acceptable alternative to the T7-RNA polymerase-generated standard amplicon), to use them as standards?
I will appreciate your help!!
pili

Ivan Delgado
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Hi pili,
I assume you are trying to study the expression of certain genes, not proteins. Single stranded oligonucleotides are synthesized by most oligo synthesis companies. For example IDT sells single stranded RNA which you could use as standards. 
 
 

pili
pili's picture
Hi Ivan:

Hi Ivan:
Thank you!!!
pili