Primer dilution

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newbie20's picture
Primer dilution

Hi all,

I am trying to figure out how to dilute my primers

My forward primer is 66.7nmol and reverse is 71.4 nmol

I know I must add 667ul and 714ul of water to the forward and reverse respectivley to make a 100uM concentration but I need a final concentration of 100 pmol of each primer in a 25ul reaction. What is the best way to dilute it down?