primer dilution

3 posts / 0 new
Last post
simer
simer's picture
primer dilution

 heya, I have stock sloution of 100 uM and In our lab we use working stock of primer as 5uM , Now, I have to made 0.7 ml of working primer? How can i make it?? Urgent reply asap, Thanks......

Ivan Delgado
Ivan Delgado's picture
You dilute your 100 uM stock

You dilute your 100 uM stock 1:20 to make it a 5 uM stock.

simer
simer's picture
ohh Thanks.. IVAN , it means

ohh Thanks.. IVAN , it means then for 20 uM , dilution will be 1:5 , for 5 uM u told it..got it now... Thanks a lot...