primer design for real time PCR

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Biopat
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primer design for real time PCR

I am designing primers for mouse RANKL but there are no god primers. My question is if anybody knows what is best of these two options : the primer has a dimer with a deltaG = -5,38 kcal/mol and a product size of 82bp or the primer has no dimer but the product size of 244 bp??

hope someone can help

Ivan Delgado
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Based on the information you provide, I would go with the second primer since it has no primer dimers and the PCR product is big enough that you do not have to worry about confusing it with a primer dimer.

Biopat
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thank you

thank you

I will try the sec. primer

vanshita
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hi, i think primer whc has no

hi, i think primer whc has no dimer u can use it. but i think u can also design ur primer manually.