Microarray and insitu hybridization

1 post / 0 new
Biolink
Biolink's picture
Microarray and insitu hybridization

 
I’m interested to understand how the brain works and I’m using fruit fly Drosophila as a model system to study the brain development. I performed microarray analysis to try to determine genes expressed in the fly brain. For my microarray experiment, I first prepared cDNA from fly brain and labeled it with a red flurochrome. Then I isolated cDNA from whole fly and labeled it with a green flurochrome. Next I hybridized these cDNA populations to a microarray containing the drosophila genes. From that I obtained a list of genes that are specifically enriched in the brain (those that show up as a red spot on the microarray).But to my disappointment my favourite gene does not appear on this list, and I repeated almost 10 times and did not encounter any technical difficulties. So I performed in situ analysis using the my favourite fly genes DNA as a probe and see that it is expressed at high levels in the fly brain of normal flies but not expressed in animals lacking my favourite gene.