96 well cold block for PCR prep

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Amtekoth
Amtekoth's picture
96 well cold block for PCR prep

I used to have one of these in an old lab, but I don't remember who makes them.

I'm looking for a metal block that you keep cold and can clean, holds 96 well plates or tubes which stay cold while preparing samples for PCR.

Ideas?

Jason King
Jason King's picture
We have blocks from Eppendorf

We have blocks from Eppendorf that are kept in the freezer and brought out on to the bench to cool 0.5mL PCR tubes and 96 well plates used for sequencing. I had a look at Eppendorf's website and they have products "Isotherm" in various formats.

I didn't see the ones we have on the site. Ours change colour as they warm up so that you know when to exchange them for cooler ones. They are supposed to keep samples cool for over 2 hours.

http://www.eppendorf.co.uk/int/index.php?l=3&sitemap=2.1&pb=d73570f6c4d7c900&action=products&contentid=1&catalognode=9859

Immunos
Immunos's picture
Biocision makes PCR cold

Biocision makes PCR cold blocks, they are form fit and allow rapid temperature change....link is http://www.biocision.com/product.php?productid=16159&cat=256&page=1 or http://www.biocision.com

R Bishop
R Bishop's picture
Yeah I saw the Biocision guys

Yeah I saw the Biocision guys at ASCB last week. It looked like it holds temperature pretty well. Not sure about the cost though?

Rb

Amtekoth
Amtekoth's picture
R Bishop wrote:Yeah I saw the

R Bishop wrote:

Yeah I saw the Biocision guys at ASCB last week. It looked like it holds temperature pretty well. Not sure about the cost though?
Rb

On the site, it said $265, which might be worth it. You can spray it with RNase-Away to protect RNA samples for RT-PCR right out of the freezer.

1.3 lbs...solid!

Amtekoth
Amtekoth's picture
parvoman wrote:

parvoman wrote:

We have blocks from Eppendorf that are kept in the freezer and brought out on to the bench to cool 0.5mL PCR tubes and 96 well plates used for sequencing. I had a look at Eppendorf's website and they have products "Isotherm" in various formats.

I didn't see the ones we have on the site. Ours change colour as they warm up so that you know when to exchange them for cooler ones. They are supposed to keep samples cool for over 2 hours.

http://www.eppendorf.co.uk/int/index.php?l=3&sitemap=2.1&pb=d73570f6c4d7c900&action=products&contentid=1&catalognode=9859

Unfortunately, they didn't have prices listed on the Eppendorf site. I like the color change feature, though.

Immunos
Immunos's picture
advantage of the pcr block

advantage of the pcr block from the biocision guys is it does not need to be pre-charged in the freezer and does keep temperature constant as long as it stays on ice and has temperature well to well consistency

cambridgevirology
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We use the BioCision blocks

We use the BioCision blocks at Novartis. They are outstanding. We use them for RT-PCR and we bought their block and trays to do protein work, RNA work and virus dilution preps and freezing down samples as well. They are solid, well made and a great assett to the lab!

Amtekoth
Amtekoth's picture
Immunos wrote:advantage of

Immunos wrote:

advantage of the pcr block from the biocision guys is it does not need to be pre-charged in the freezer and does keep temperature constant as long as it stays on ice and has temperature well to well consistency

You need to keep the block on ice? I hate having ice anywhere near my open RNA tubes. (just a little paranoid about RNAses.

cambridgevirology
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Not really, the cool thing

Not really, the cool thing about Biocision's product is that you can place the plate on ice so that the tubes are not in contact with the ice but they are kep at 4C. In drug development we are used to large number of tubes and we solve the RNA degradation problem by buying their trays and keeping the plates on top of the plate and the tubes keep at 4C. They are called ThermalTrays I believe, check them out!

Immunos
Immunos's picture
problem with plastic racks

problem with plastic racks and plastic coolers for this app is that they are poor thermoconductors hence when taken out of the freezer they are like a turkey out of freezer they get warm first outside while they are still very cold inside...not a good thing for handling DNA, RNA etc...esp w/ PCR

martin wang
martin wang's picture
A model of cooling, heating

A model of cooling, heating block manufactured by AMPICON ThermoMix500 may work for you. Set it at 4C and it will always be cold! No ice and no need tor pre chilling.

TheFFM
TheFFM's picture
 Biocision have now

 Biocision have now eliminated the ice factor and have the CoolBox in which you can keep the 96 well CoolRack.

You just keep an ice pack which is wrapped in a metal case in the freezer and place it in the cool box when you are ready to start your experiment and them place the 96 well CoolTack on top of that and it chills down and stays chilled for hours with the lid on.

I'm using it to keep my RNA cold for oocyte injections right now.

I'll be using it to assemble my PCR reactions in the not to distant future.

This had been a great new little toy to have on the bench recently because our departmental ice machine broke in April and still is not fixed!

cgumm22
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I know Light Labs has one

I know Light Labs has one that is common in labs.  Is this what you are looking for?

www.lightlabsusa.com/96-Well-Aluminum-Block.html

Amtekoth
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I've never heard of Light

I've never heard of Light Labs before, but that looks like the sort of rack that could work.  $65 each is affordable, as well.

James M
James M's picture
 For cooling PCR trays/ tubes

 For cooling PCR trays/ tubes Pioneer Scientific have a great product. PCR Cooling chambers. They are made of solid aluminium and have additional space to cool 2ml, and 0.5 ml reagent tubes while preparing PCR reagrents. They also have a PCR Double Cooling Chamber where you can prepare two PCR trays at once. This is a great product and is used by a lot of people I know for PCR preparation. 

James M
James M's picture
PCR Cooling Chamber from

PCR Cooling Chamber from Pioneer Scientific

Chris Faulkner
Chris Faulkner's picture
PCR Cooler Block from Lab Wholesale

http://labwholesale.com/lab/catalogsearch/result/?q=coolersciI found these on labwholesale.com from Cooler Scientific and they work well for our lab.  At a great price - better than many of the other options on this thread.