Our lab is looking to develop a comet assay to assess DNA damage induced by ionizing radiation. So we are looking for double stranded DNA breaks. Any help would be appreciated.
There are a few comet assay protocols here:www.scientistsolutions.com/science-protocols_protocolslist_84bf041c-2927-41e3-b971-56af59672b4b.aspx
Don't know if they are exactly what you are looking for, but maybe they will help.
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Thanks Omai, these comet assays are a funny thing. Its so hard to nail down sometimes. I think you spend most of the time looking at the monitor and asking "is that a comet". I will pass these on, and hopefully we can start seeing comets instead of moons!
Thanks for your input into the comet assay. We are using a neutral comet assay to detect DNA double strand breaks. We are creating DNA double strand breaks with ionizing radiation. We see comets, but we are also seeing them in the control non treated cells. Would anyone have any suggestions. The protocols online have led us to see comets, now we are just trying to optimize it.
I am also looking for a good protocol for the Neutral COMET assay. Alkalines work well but with the Trevigen Protocol for Neutral COMET we don't see any COMETs even in the positive control. Can anyone provide me with their protocol!